Application
Western blot analysis on (A) A431, (B) HeLa, and (C) SW480 cell lysates using anti-MSH6 RabMAb (Cat. #AJ1510a).Application
Immunohistochemical analysis of paraffin-embedded human colonic adenocarcinoma tissue using anti-MSH6 RabMAb (Cat. #AJ1510a).Application
Immunofluorescent staining of HeLa cells using anti-MSH6 RabMAb (Cat. #AJ1510a).| Product Name | MSH6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3945 |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GTBP |
|---|---|
| Molecular Weight(MW) | 152786 Da |
| Function | Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair |
| Tissue Specificity | A synthetic peptide corresponding to residues at the N-terminus in human MSH6 was used as an immunogen. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 2956 |
|---|
Application
Western blot analysis on (A) A431, (B) HeLa, and (C) SW480 cell lysates using anti-MSH6 RabMAb (Cat. #AJ1510a).
Application
Immunohistochemical analysis of paraffin-embedded human colonic adenocarcinoma tissue using anti-MSH6 RabMAb (Cat. #AJ1510a).
Application
Immunofluorescent staining of HeLa cells using anti-MSH6 RabMAb (Cat. #AJ1510a).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
