Application
Figure 1: Western blot analysis using anti-MSH6 monoclonal antibody against truncated MSH6 recombinant protein.| Product Name | MSH6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GTBP; HSAP; HNPCC5 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3A10H7 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500 - 2000. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GTBP |
|---|---|
| Alternative Names | GTBP HSAP HNPCC5 |
| Molecular Weight(MW) | 152786 Da |
| Function | Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair |
| Tissue Specificity | Purified recombinant fragment of MSH6 expressed in E. Coli. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 2956 |
|---|---|
| Nucleotide Accession | NP_000170.1 |
Application
Figure 1: Western blot analysis using anti-MSH6 monoclonal antibody against truncated MSH6 recombinant protein.| Application Notes | WB:1:500 - 2000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of MSH6 expressed in E. Coli. |
