Application
Figure 1: Immunohistochemical analysis of paraffin-embedded human lung cancer (A), muscles (B) and breast cancer (C) using anti-MUSK antibody with DAB staining.Application
Figure 2: Confocal immunofluorescence analysis of HEK293 cells trasfected with extracellular MUSK (aa24-209)-hIgGFc using anti-MUSK monoclonal antioby (green). Blue: DRAQ5 fluorescent DNA dye.| Product Name | MUSK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | muscle, skeletal, receptor tyrosine kinase |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 10A4 |
| Host Species | Mouse |
| Tested Applications | IHCIF |
| IHC:1:200 - 1000. IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | muscle skeletal receptor tyrosine kinase |
|---|---|
| Molecular Weight(MW) | 97056 Da |
| Function | Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between the motor neuron and the skeletal muscle. Recruitment of AGRIN by LRP4 to the MUSK signaling complex induces phosphorylation and activation of MUSK, the kinase of the complex. The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic membrane May regulate AChR phosphorylation and clustering through activation of ABL1 and Src family kinases which in turn regulate MUSK. DVL1 and PAK1 that form a ternary complex with MUSK are also important for MUSK-dependent regulation of AChR clustering. May positively regulate Rho family GTPases through FNTA. Mediates the phosphorylation of FNTA which promotes prenylation, recruitment to membranes and activation of RAC1 a regulator of the actin cytoskeleton and of gene expression. Other effectors of the MUSK signaling include DNAJA3 which functions downstream of MUSK. May also play a role within the central nervous system by mediating cholinergic responses, synaptic plasticity and memory formation (By similarity) |
| Tissue Specificity | Purified recombinant extracellular fragment of human MUSK (aa24-209) fused with hIgGFc tag expressed in HEK293 cell line. |
| Cellular Localization | Cell junction, synapse, postsynaptic cell membrane; Single-pass type I membrane protein (Probable) Note=Localizes to the postsynaptic cell membrane of the neuromuscular junction (Probable) |
| Entrez Gene | 4593 |
|---|---|
| Nucleotide Accession | NP_005583.1 |
Application
Figure 1: Immunohistochemical analysis of paraffin-embedded human lung cancer (A), muscles (B) and breast cancer (C) using anti-MUSK antibody with DAB staining.
Application
Figure 2: Confocal immunofluorescence analysis of HEK293 cells trasfected with extracellular MUSK (aa24-209)-hIgGFc using anti-MUSK monoclonal antioby (green). Blue: DRAQ5 fluorescent DNA dye.| Application Notes | IHC:1:200 - 1000. IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant extracellular fragment of human MUSK (aa24-209) fused with hIgGFc tag expressed in HEK293 cell line. |
