Application
Western blot analysis of anti-NANOG monoclonal antibody (Cat.#AM1486b) by NONAG recombinant protein. NANOG (NANOG + His tag)(arrow) was detected using the Mab.Application
NANOG (Cat. #AM1486b) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the NANOG antibody detected the NANOG protein (arrow).Application
NANOG Antibody (Cat. #AM1486b)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NANOG Antibody for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | NANOG Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NANOG; Homeobox protein NANOG; Homeobox transcription factor Nanog |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 60CT77.1.1 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:100~250 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | NANOG Homeobox protein NANOG Homeobox transcription factor Nanog |
|---|---|
| Molecular Weight(MW) | 34620 Da |
| Function | Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'- [CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation |
| Tissue Specificity | Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes |
| Cellular Localization | Nucleus. |
| Entrez Gene | 79923 |
|---|---|
| Nucleotide Accession | NP_079141.2 XP_002344676.1 |
Application
Western blot analysis of anti-NANOG monoclonal antibody (Cat.#AM1486b) by NONAG recombinant protein. NANOG (NANOG + His tag)(arrow) was detected using the Mab.
Application
NANOG (Cat. #AM1486b) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the NANOG antibody detected the NANOG protein (arrow).
Application
NANOG Antibody (Cat. #AM1486b)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NANOG Antibody for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~250 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
