Application
Figure 1: Western blot analysis using anti-NANOG monoclonal antibody against NTERA-2 cell lysate (2).Application
Figure 2: Confocal immunofluorescence analysis of NTERA-2 cells (left) and HeLa cells (right) using anti-Nanog Mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.| Product Name | NANOG Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NANOG |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1E6C4 |
| Host Species | Mouse |
| Tested Applications | WBIF |
| WB:1:500 - 2000. IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | NANOG |
|---|---|
| Molecular Weight(MW) | 34620 Da |
| Function | Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'- [CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation |
| Tissue Specificity | Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes |
| Cellular Localization | Nucleus. |
| Entrez Gene | 79923 |
|---|---|
| Nucleotide Accession | XP_002344676.1 |
Application
Figure 1: Western blot analysis using anti-NANOG monoclonal antibody against NTERA-2 cell lysate (2).
Application
Figure 2: Confocal immunofluorescence analysis of NTERA-2 cells (left) and HeLa cells (right) using anti-Nanog Mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.| Application Notes | WB:1:500 - 2000. IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of NANOG (aa20-166) expressed in E. Coli. |
