Application
Western blot analysis of NDEL1 Antibody (Center) in A375 cell line lysates (35ug/lane). NDEL1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain reacted with NDEL1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of NDEL1 (arrow) using rabbit polyclonal NDEL1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NDEL1 gene.Application
NDEL1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | NDEL1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NDEL1; EOPA; MITAP1; NUDEL; Nuclear distribution protein nudE-like 1; Mitosin-associated protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EOPA MITAP1 NUDEL |
|---|---|
| Alternative Names | NDEL1 EOPA MITAP1 NUDEL Nuclear distribution protein nudE-like 1 Mitosin-associated protein 1 |
| Molecular Weight(MW) | 38375 Da |
| Function | Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL |
| Tissue Specificity | Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle |
| Cellular Localization | Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Chromosome, centromere, kinetochore Cytoplasm, cytoskeleton, spindle. Note=Localizes to the cell body of the motor neurons and colocalizes with assembled neurofilaments within axonal processes. Localizes to the microtubules of the manchette in elongated spermatids. Colocalizes with DISC1 in the perinuclear region, including the centrosome (By similarity) Localizes to the interphase centrosome and the mitotic spindle Localizes to the kinetochore in a CENPF-dependent manner |
| Entrez Gene | 81565 |
|---|
Application
Western blot analysis of NDEL1 Antibody (Center) in A375 cell line lysates (35ug/lane). NDEL1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain reacted with NDEL1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of NDEL1 (arrow) using rabbit polyclonal NDEL1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NDEL1 gene.
Application
NDEL1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
