Application
Western blot analysis of anti-Phospho-Nudel-pS231 Pab in CEM cell line tissue lysate (35ug/lane).Phospho-Nudel-pS231(arrow) was detected using the purified Pab (1:2220 dilution).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | Phospho-Nudel-S231 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NDEL1; EOPA; MITAP1; NUDEL; Nuclear distribution protein nudE-like 1; Mitosin-associated protein 1 |
| Modification | p-S231 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EOPA MITAP1 NUDEL |
|---|---|
| Alternative Names | NDEL1 EOPA MITAP1 NUDEL Nuclear distribution protein nudE-like 1 Mitosin-associated protein 1 |
| Molecular Weight(MW) | 38375 Da |
| Function | Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL |
| Tissue Specificity | Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle |
| Cellular Localization | Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Chromosome, centromere, kinetochore Cytoplasm, cytoskeleton, spindle. Note=Localizes to the cell body of the motor neurons and colocalizes with assembled neurofilaments within axonal processes. Localizes to the microtubules of the manchette in elongated spermatids. Colocalizes with DISC1 in the perinuclear region, including the centrosome (By similarity) Localizes to the interphase centrosome and the mitotic spindle Localizes to the kinetochore in a CENPF-dependent manner |
Application
Western blot analysis of anti-Phospho-Nudel-pS231 Pab in CEM cell line tissue lysate (35ug/lane).Phospho-Nudel-pS231(arrow) was detected using the purified Pab (1:2220 dilution).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
