Application
NDST1 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the NDST1 antibody detected the NDST1 protein (arrow).Application
NDST1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NDST1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | NDST1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NDST1; HSST; HSST1; Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1; Glucosaminyl N-deacetylase/N-sulfotransferase 1; N-heparan sulfate sulfotransferase 1; [Heparan sulfate]-glucosamine N-sulfotransferase 1; Heparan sulfate N-deacetylase 1; |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HSST HSST1 |
|---|---|
| Alternative Names | NDST1 HSST HSST1 Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1 Glucosaminyl N-deacetylase/N-sulfotransferase 1 N-heparan sulfate sulfotransferase 1 [Heparan sulfate]-glucosamine N-sulfotransferase 1 Heparan sulfate N-deacetylase 1 |
| Molecular Weight(MW) | 100868 Da |
| Function | Essential bifunctional enzyme that catalyzes both the N- deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L- selectin ligands, thereby playing a role in inflammatory response |
| Tissue Specificity | Widely expressed. Expression is most abundant in heart, liver and pancreas |
| Cellular Localization | Golgi apparatus membrane; Single-pass type II membrane protein |
| Entrez Gene | 3340 |
|---|---|
| Nucleotide Accession | NP_001534.1 |
Application
NDST1 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the NDST1 antibody detected the NDST1 protein (arrow).
Application
NDST1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NDST1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
