WB
Western blot analysis of NDST1 expression in CEM (A) whole cell lysates.IHC
Immunohistochemical analysis of NDST1 staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Product Name | Anti-NDST1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human NDST1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:1000 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NDST1 |
|---|---|
| Gene Synonyms | HSST NST1 MRT46 |
| Gene Full Name | N-deacetylase and N-sulfotransferase 1 |
| Gene Summary | This gene encodes a member of the heparan sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family. The encoded enzyme is a type II transmembrane protein that resides in the Golgi apparatus. The encoded protein catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to nitrogen of glucosamine in heparan sulfate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| Alternative Names | HSST HSST1 Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1 Glucosaminyl N-deacetylase/N-sulfotransferase 1 NDST-1 N-heparan sulfate sulfotransferase 1 N-HSST 1 [Heparan sulfate]-glucosamine N-sulfotransferase 1 HSNST 1 |
| Molecular Weight(MW) | 100 kDa(Observed band size: 120 kDa) |
| Cellular Localization | Golgi apparatus,Membrane. |
WB
Western blot analysis of NDST1 expression in CEM (A) whole cell lysates.
IHC
Immunohistochemical analysis of NDST1 staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Application Notes | WB:1:500-1:1000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
