Application
Western blot analysis of NEFL antibody (Center R185) in Y79 cell line lysates (35ug/lane). NEFL (arrow) was detected using the purified Pab.Application
NEFL Antibody (Center R185) (RB18878) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NEFL Antibody (Center R185) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of NEFL Antibody (Center R185) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | NEFL Antibody (Center R185) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NEFL; NF68; NFL; Neurofilament light polypeptide; 68 kDa neurofilament protein; Neurofilament triplet L protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NF68 NFL |
|---|---|
| Alternative Names | NEFL NF68 NFL Neurofilament light polypeptide 68 kDa neurofilament protein Neurofilament triplet L protein |
| Molecular Weight(MW) | 61517 Da |
| Function | Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber |
| Tissue Specificity | This NEFL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177~207 amino acids from the Center region of human NEFL. |
| Entrez Gene | 4747 |
|---|
Application
Western blot analysis of NEFL antibody (Center R185) in Y79 cell line lysates (35ug/lane). NEFL (arrow) was detected using the purified Pab.
Application
NEFL Antibody (Center R185) (RB18878) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NEFL Antibody (Center R185) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of NEFL Antibody (Center R185) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
