Application
Formalin fixed paraffin embedded human cerebellum stained with Neurofilament antibody .| Product Name | NF-H + NF-L antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [2F11] to NF-H + NF-LNeurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. |
| Immunogen | Purified neurofilaments from human brain |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2F11 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-P |
| For IHC-Fr: Pretitred for use on acentone fixed frozen tissue sections. No further titration is required. For IHC-P: Pretitred for use on formalin-fixed paraffin embedded tissue sections. No further titration is required. Incuabte 30 minutes at RT. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseDogPigRatCatHorseOpossumRabbitBovine |
| Concentration | 1mg/ml |
| Gene Synonyms | NF68 Nfh CMT2E NFL NF-H Nefh 18039 380684 Neurofilament NF200 NF-L AI847934 mKIAA0845 Nefl NF H NF L |
|---|---|
| Target | NF-H + NF-L |
| Tissue Specificity | Reacts with 70 and 200 kD components of the polypeptide subunit. It stains neurons, neuronal processes and peripheral nerves. This antibody reacts with some neuroblastomas and gangliomas but not with other non-neuronal tumors. |
| Cellular Localization | Cytoplasmic |
| Light Chain Type | kappa |
Application
Formalin fixed paraffin embedded human cerebellum stained with Neurofilament antibody .| Positive Control | Brain |
|---|---|
| Application Notes | For IHC-Fr: Pretitred for use on acentone fixed frozen tissue sections. No further titration is required. For IHC-P: Pretitred for use on formalin-fixed paraffin embedded tissue sections. No further titration is required. Incuabte 30 minutes at RT. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline containing sodium azide |
