| Product Name | NF-H antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [RT97] to NF-HNeurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appear as 10nm diameter fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. They are composed a mixture of subunits which usually include the three neurofilament triplet proteins known as NFL, NFM and NFH. Neurofilaments may also include smaller amounts of peripherin, alpha internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable. GTX74883 stains neuromas, gangliomas, neuroblastomas and medulloblastomas. It also stains neuronal processes in brain and other nervous tissues. It is useful to detect paragangliomas, phaeochromocytomas and other tumors of neuroectodermal origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | RT97 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-P |
| For IHC-Fr and IHC-P: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | Nfh |
|---|---|
| Target | NF-H |
| Tissue Specificity | recognises the 200kD neurofilament protein in a range of species. The antibody stains various tumours including phaeochromocytoma, paraganglioma and ganglioneuroblastoma.It has also been demonstrated that also recognises a phosphorylation dependent epitope on microtubule associated protein 1B (MAP1B) and on Tau in Western blots. Reactivity of the antibody with MAP1B and Tau is not seen under the conditions for immunohistochemistry. |
| Positive Control | Brain or spinal cord |
|---|---|
| Application Notes | For IHC-Fr and IHC-P: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline containing 10 mM sodium azide |
