Application
Immunocytochemistry/Immunofluorescence: 160kDa Neurofilament Medium Antibody [ - Section of rat cerebral cortex stained with (red), which reveals the perikarya of pyramidal neurons and dendrites and axons surrounding them. The green channel shows staining with a monoclonal antibody to the beta-adrendergic receptor kinase 1.Application
Western Blot: 160kDa Neurofilament Medium Antibody [ - Western blot analysis of 160kDa Neurofilament Medium expression on whole rat cerebellum homogenate using| Product Name | NF-M antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NF-MNeurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-M is the middle or medium neurofilament subunit and runs on SDS-PAGE gels in the range 145-170kDa, with some variation in different species. Antibodies to NF-M are useful to identify this protein and identify neurons and their processes in tissue sections and in tissue culture. NF-M can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease. |
| Immunogen | Recombinant C-terminal segment of rat NF-M |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FIHC-PWB |
| Can be used on cryostat sections and formalin-fixed paraffin-embedded histological sections. Suggested working dilutions: Western Blot 1:5000 Immunohistochemistry 1:1000 The investigator should determine the optimal dilution for a specific application.: |
|
| Species Reactivity | HumanMousePigRatBirdCatMammalian speciesReptilesBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Nfh |
|---|---|
| Target | NF-M |
| Tissue Specificity | Specifically recognizes the evolutionarily conserved extreme C-terminal region of the medium neurofilament subunit (~145-170kDa). |
Application
Immunocytochemistry/Immunofluorescence: 160kDa Neurofilament Medium Antibody [ - Section of rat cerebral cortex stained with (red), which reveals the perikarya of pyramidal neurons and dendrites and axons surrounding them. The green channel shows staining with a monoclonal antibody to the beta-adrendergic receptor kinase 1.
Application
Western Blot: 160kDa Neurofilament Medium Antibody [ - Western blot analysis of 160kDa Neurofilament Medium expression on whole rat cerebellum homogenate using| Application Notes | Can be used on cryostat sections and formalin-fixed paraffin-embedded histological sections. Suggested working dilutions: Western Blot 1:5000 Immunohistochemistry 1:1000 The investigator should determine the optimal dilution for a specific application.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
