Application
A. Western blot analysis on SH-SY5Y cell lysates using anti- NF-M (pS614/619) RabMAb (Cat. #AJ1532b), 1:500 dilution.Application
B. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-NF-M RabMAb (Cat. #AJ1532b).| Product Name | Neurofilament M Antibody Phospho (pS614/619) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NEFM, NEF3, NFM, Neurofilament medium polypeptide, Neurofilament triplet M protein;160 kDa neurofilament protein;Neurofilament 3 |
| Modification | p-S614/619 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR580(2)Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NEF3 NFM |
|---|---|
| Alternative Names | NEFM NEF3 NFM Neurofilament medium polypeptide Neurofilament triplet M protein 160 kDa neurofilament protein Neurofilament 3 |
| Molecular Weight(MW) | 102472 Da |
| Function | Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber |
| Tissue Specificity | A synthetic phospho-peptide corresponding to residues surrounding Serine 614/619 of human NF-M was used as an immunogen. |
| Entrez Gene | 4741 |
|---|
Application
A. Western blot analysis on SH-SY5Y cell lysates using anti- NF-M (pS614/619) RabMAb (Cat. #AJ1532b), 1:500 dilution.
Application
B. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-NF-M RabMAb (Cat. #AJ1532b).| Application Notes | WB:1:500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
