Application
A. Western blot analysis on (A) Hu-placenta (B) HUVEC (C) HepG2 cell lysates (D) mouse heart tissue (E) mouse kidney tissue (F) rat heart and (G) rat kidney tissue lysate using anti-Neuropilin-1 RabMAb (Cat. #AJ1534a), dilution 1:1000Application
B. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Neuropilin 1 RabMAb (Cat. #AJ1534a).| Product Name | Neuropilin-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PKM2, PK2, PK3, PKM, Pyruvate kinase isozymes M1/M2, THBP1;p58;M2-PK;Tumor M2-PK |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3113 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NRP VEGF165R |
|---|---|
| Alternative Names | PKM2 PK2 PK3 PKM Pyruvate kinase isozymes M1/M2 THBP1 p58 M2-PK Tumor M2-PK |
| Molecular Weight(MW) | 103134 Da |
| Function | The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis |
| Tissue Specificity | The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein |
| Entrez Gene | 8829 |
|---|
Application
A. Western blot analysis on (A) Hu-placenta (B) HUVEC (C) HepG2 cell lysates (D) mouse heart tissue (E) mouse kidney tissue (F) rat heart and (G) rat kidney tissue lysate using anti-Neuropilin-1 RabMAb (Cat. #AJ1534a), dilution 1:1000
Application
B. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Neuropilin 1 RabMAb (Cat. #AJ1534a).| Application Notes | WB:1:1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Data sheet for OM215245