WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Optineurin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.IHC
Human colon carcinoma was stained with Anti-Optineurin rabbit antibody.IHC
Human brain was stained with Anti-Optineurin rabbit antibody.ICC/IF
Immunofluorescence analysis of HEK293. Picture A:Optineurin antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.IF-P
mouse brain was stained with Anti-Optineurin rabbit antibody.| Product Name | Optineurin Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:1000-5000 IHC:1:200-500 ICC/IF:1:200-1:1000 IF-P:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | OPTN |
|---|---|
| Gene Synonyms | NRP FIP2 HIP7 HYPL ALS12 GLC1E TFIIIA-INTP |
| Gene Full Name | optineurin |
| Gene Summary | This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 63kDa(Observed band size: 74kDa) |
| Cellular Localization | Cytoplasm. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Optineurin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
IHC
Human colon carcinoma was stained with Anti-Optineurin rabbit antibody.
IHC
Human brain was stained with Anti-Optineurin rabbit antibody.
ICC/IF
Immunofluorescence analysis of HEK293. Picture A:Optineurin antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.
IF-P
mouse brain was stained with Anti-Optineurin rabbit antibody.| Application Notes | WB:1:1000-5000 IHC:1:200-500 ICC/IF:1:200-1:1000 IF-P:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
Data sheet for OM644277