Application
Western blot analysis on (A) Jurkat and (B) Ramos cell lysates using anti-NFAT1 RabMAb (Cat. #AJ1536a).| Product Name | NFAT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NFATC2, NFAT1, NFATP, Nuclear factor of activated T-cells, cytoplasmic 2, T-cell transcription factor NFAT1;NFAT pre-existing subunit |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR2973 |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000~5000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NFAT1 NFATP |
|---|---|
| Alternative Names | NFATC2 NFAT1 NFATP Nuclear factor of activated T-cells cytoplasmic 2 T-cell transcription factor NFAT1 NFAT pre-existing subunit |
| Molecular Weight(MW) | 100146 Da |
| Function | Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway |
| Tissue Specificity | Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas. Isoform 1 is highly expressed in the small intestine, heart, testis, prostate, thymus, placenta and thyroid. Isoform 3 is highly expressed in stomach, uterus, placenta, trachea and thyroid |
| Cellular Localization | Cytoplasm. Nucleus. Note=Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals The subcellular localization of NFATC plays a key role in the regulation of gene transcription |
| Entrez Gene | 4773 |
|---|
Application
Western blot analysis on (A) Jurkat and (B) Ramos cell lysates using anti-NFAT1 RabMAb (Cat. #AJ1536a).| Application Notes | WB:1:1000~5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
