Application
Immunofluorescence of monoclonal antibody to NFIC (AT3033a) on HeLa cell. [antibody concentration 10ug/ml]Application
Western blot analysis of NFIC over-expressed 293 cell line, cotransfected with NFIC Validated Chimera RNAi ( Cat # H00004782-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NFIC monoclonal antibody (M01), clone 1C12-2A2 (Cat # AT3033a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.| Product Name | NFIC Antibody (monoclonal) (M01) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CTF, CTF5, MGC20153, NFI, NFInuclear factor I/C (CCAAT-binding transcription factor) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1C12-2A2 |
| Host Species | Mouse |
| Tested Applications | IFWB |
| WB:1:500~1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CTF CTF5 MGC20153 NFI NFInuclear factor I/C (CCAAT-binding transcription factor) |
|---|---|
| Molecular Weight(MW) | 55675 Da |
| Tissue Specificity | NFIC (AAH12120, 1 a.a. ~ 429 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Entrez Gene | 4782 |
|---|
Application
Immunofluorescence of monoclonal antibody to NFIC (AT3033a) on HeLa cell. [antibody concentration 10ug/ml]
Application
Western blot analysis of NFIC over-expressed 293 cell line, cotransfected with NFIC Validated Chimera RNAi ( Cat # H00004782-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NFIC monoclonal antibody (M01), clone 1C12-2A2 (Cat # AT3033a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.| Application Notes | WB:1:500~1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
