Application
Western blot analysis of NGLY1 antibody (Center) in mouse kidney tissue lysates (35ug/lane). NGLY1 (arrow) was detected using the purified Pab.Application
(LEFT)Formalin-fixed and paraffin-embedded human brain tissue reacted with NGLY1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Flow cytometric analysis of 293 cells using NGLY1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | NGLY1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PNG1 |
|---|---|
| Alternative Names | NGLY1 PNG1 Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase N-glycanase 1 Peptide:N-glycanase |
| Molecular Weight(MW) | 74390 Da |
| Function | Specifically deglycosylates the denatured form of N- linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl- glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high- mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins |
| Tissue Specificity | This NGLY1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250~280 amino acids from the C-terminal region of human XPO6. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 55768 |
|---|
Application
Western blot analysis of NGLY1 antibody (Center) in mouse kidney tissue lysates (35ug/lane). NGLY1 (arrow) was detected using the purified Pab.
Application
(LEFT)Formalin-fixed and paraffin-embedded human brain tissue reacted with NGLY1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Flow cytometric analysis of 293 cells using NGLY1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
