WB
Loading:Approximately 3 μg of total proteins from Arabidopsis thaliana and rice leaves Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Product Name | Anti-PNG1/ Peptide:N-glycanase |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT5G49570，Q9FGY9 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:10000 |
|
| Species Reactivity | ArabidopsisRice |
| Concentration | 1μg/μl |
| Purification | Affinity purified |
| Gene Symbol | PNG1 |
|---|---|
| Gene Synonyms | ATPNG1, PEPTIDE-N-GLYCANASE 1, PNG1，Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase |
| Gene Full Name | PNG1/ Peptide:N-glycanase |
| Gene Summary | PNG1 encodes a hydrolase protein that has peptide:N-glycanase activity in enzymatic assay in heterologous systems. It specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. |
| Molecular Weight(MW) | 82.4 kDa |
WB
Loading:Approximately 3 μg of total proteins from Arabidopsis thaliana and rice leaves Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Application Notes | WB:1:2500-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 50% glycerol，-20 °C |
