Application
Western blot analysis of NKRF antibody (Center) in Jurkat cell line lysates (35ug/lane). NKRF (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain reacted with NKRF Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | NKRF Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ITBA4, NRF |
|---|---|
| Alternative Names | NKRF ITBA4 NRF NF-kappa-B-repressing factor Protein ITBA4 Transcription factor NRF |
| Molecular Weight(MW) | 77673 Da |
| Function | Interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity. Also involved in the regulation of IL-8 transcription |
| Tissue Specificity | Widely and constitutively expressed. Expressed at lower level in colon, peripheral blood lymphocytes, lung and kidney |
| Cellular Localization | Nucleus, nucleolus. |
| Entrez Gene | 55922 |
|---|
Application
Western blot analysis of NKRF antibody (Center) in Jurkat cell line lysates (35ug/lane). NKRF (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain reacted with NKRF Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
