Application
Western blot analysis of anti-NRAS Antibody (N-term) in CEM cell line lysates (35ug/lane). NRAS(arrow) was detected using the purified Pab.Application
Western blot analysis of NRAS (arrow) using rabbit polyclonal NRAS Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NRAS gene (Lane 2) (Origene Technologies).Application
NRAS Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of NRAS Antibody (N-term)(Cat#AP7745a) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Product Name | NRAS Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NRAS; HRAS1; GTPase NRas; Transforming protein N-Ras |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFCIF |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HRAS1 |
|---|---|
| Alternative Names | NRAS HRAS1 GTPase NRas Transforming protein N-Ras |
| Molecular Weight(MW) | 21229 Da |
| Function | Ras proteins bind GDP/GTP and possess intrinsic GTPase activity |
| Tissue Specificity | This NRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79~109 amino acids from the N-terminal region of human NRAS. |
| Cellular Localization | Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor. Note=Shuttles between the plasma membrane and the Golgi apparatus |
| Entrez Gene | 4893 |
|---|
Application
Western blot analysis of anti-NRAS Antibody (N-term) in CEM cell line lysates (35ug/lane). NRAS(arrow) was detected using the purified Pab.
Application
Western blot analysis of NRAS (arrow) using rabbit polyclonal NRAS Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NRAS gene (Lane 2) (Origene Technologies).
Application
NRAS Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of NRAS Antibody (N-term)(Cat#AP7745a) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
