Application
A. Western blot analysis on MCF7 cell lysate using anti-Ras/p21 RabMAb (Cat. #AJ1568b), dilution 1:500.Application
B. Immunohistochemical staining of HeLa cells using anti- Ras/p21 RabMAb (Cat. #AJ1568b)| Product Name | p21/Ras Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NRAS, HRAS1, GTPase NRas, Transforming protein N-Ras |
| Clonality | Monoclonal |
|---|---|
| Clone Number | Y131 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:2000~10000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HRAS1 |
|---|---|
| Alternative Names | NRAS HRAS1 GTPase NRas Transforming protein N-Ras |
| Molecular Weight(MW) | 21229 Da |
| Function | Ras proteins bind GDP/GTP and possess intrinsic GTPase activity |
| Tissue Specificity | A synthetic peptide corresponding to residues in the effector region of human Ras was used as an immunogen. Predicted to react with other Ras family members and cross-react with most species based on sequence homology. |
| Cellular Localization | Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor. Note=Shuttles between the plasma membrane and the Golgi apparatus |
| Entrez Gene | 4893 |
|---|
Application
A. Western blot analysis on MCF7 cell lysate using anti-Ras/p21 RabMAb (Cat. #AJ1568b), dilution 1:500.
Application
B. Immunohistochemical staining of HeLa cells using anti- Ras/p21 RabMAb (Cat. #AJ1568b)| Application Notes | WB:1:2000~10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
