Application
Western blot analysis of NUCL Antibody (Cat. #AM1824a) in 293 cell line lysates (35ug/lane). NUCL (arrow) was detected using the purified Mab.Application
NUCL Monoclonal Antibody (Cat. #AM1824A) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NUCL Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of NUCL Antibody(Cat#AM1824a) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | NUCL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 130CT43.8.5 |
| Host Species | Mouse |
| Tested Applications | WBIHCIF |
| WB:1:500~1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | NCL Nucleolin Protein C23 |
|---|---|
| Molecular Weight(MW) | 76614 Da |
| Function | Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats |
| Tissue Specificity | This NUCL Monoclonal antibody is generated from mouses immunized with a protein from human NUCL. |
| Cellular Localization | Nucleus, nucleolus. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs |
| Entrez Gene | 4691 |
|---|
Application
Western blot analysis of NUCL Antibody (Cat. #AM1824a) in 293 cell line lysates (35ug/lane). NUCL (arrow) was detected using the purified Mab.
Application
NUCL Monoclonal Antibody (Cat. #AM1824A) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NUCL Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of NUCL Antibody(Cat#AM1824a) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:500~1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
