Application
Western blot analysis of anti- NUP54 Antibody (Cat. #AM1830b) in K562 cell line lysates (35ug/lane). NUP54 (arrow) was detected using the purified Mab.Application
NUP54 Monoclonal Antibody (Cat. #AM1830b) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NUP54 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | NUP54 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 139CT1.1.5 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | NUP54 Nucleoporin p54 54 kDa nucleoporin |
|---|---|
| Molecular Weight(MW) | 55435 Da |
| Function | Component of the nuclear pore complex, a complex required for the trafficking across the nuclear membrane (By similarity) |
| Tissue Specificity | This NUP54 Monoclonal antibody is generated from mouse immunized with NUP54 recombinant protein. |
| Cellular Localization | Nucleus, nuclear pore complex (By similarity). Nucleus membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side (By similarity). Note=Biased towards cytoplasmic side. Central region of the nuclear pore complex, within the transporter (By similarity) |
| Entrez Gene | 53371 |
|---|
Application
Western blot analysis of anti- NUP54 Antibody (Cat. #AM1830b) in K562 cell line lysates (35ug/lane). NUP54 (arrow) was detected using the purified Mab.
Application
NUP54 Monoclonal Antibody (Cat. #AM1830b) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NUP54 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
