Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with NUP98 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of NUP98 Antibody (C-term) in Hela cell line lysates (35ug/lane). NUP98 (arrow) was detected using the purified Pab.Application
Flow cytometric analysis of hela cells using NUP98 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | NUP98 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWBFC |
| IHC:1:50~100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ADAR2 |
|---|---|
| Alternative Names | NUP98 ADAR2 Nuclear pore complex protein Nup98-Nup96 Nuclear pore complex protein Nup98 98 kDa nucleoporin Nucleoporin Nup98 Nuclear pore complex protein Nup96 96 kDa nucleoporin Nucleoporin Nup96 |
| Molecular Weight(MW) | 197579 Da |
| Function | Nup98 and Nup96 play a role in the bidirectional transport across the nucleoporin complex (NPC). The FG repeat domains in Nup98 have a direct role in the transport |
| Tissue Specificity | This NUP98 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1194~1223 amino acids from the C-terminal region of human NUP98. |
| Cellular Localization | Nucleus, nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side Note=Nup96 is localized to the nucleoplasmic side of the nuclear pore complex, at or near the nucleoplasmic basket |
| Entrez Gene | 4928 |
|---|
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with NUP98 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of NUP98 Antibody (C-term) in Hela cell line lysates (35ug/lane). NUP98 (arrow) was detected using the purified Pab.
Application
Flow cytometric analysis of hela cells using NUP98 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | IHC:1:50~100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
