Application
The anti-OAS1 Pab is used in Western blot to detect OAS1 in mouse liver lysate.Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with OAS1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | OAS1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | OIAS |
|---|---|
| Alternative Names | OAS1 OIAS 2'-5'-oligoadenylate synthase 1 E18/E16 p46/p42 OAS |
| Molecular Weight(MW) | 46029 Da |
| Function | Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. The secreted form displays antiviral effect against vesicular stomatitis virus (VSV), herpes simplex virus type 2 (HSV-2), and encephalomyocarditis virus (EMCV) and stimulates the alternative antiviral pathway independent of RNase L |
| Tissue Specificity | This OAS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309~339 amino acids selected from the C-terminal region of human OAS1. |
| Cellular Localization | Cytoplasm. Mitochondrion. Nucleus. Microsome. Endoplasmic reticulum. Secreted (By similarity) Note=Associated with different subcellular fractions such as mitochondrial, nuclear, and rough/smooth microsomal fractions |
| Entrez Gene | 4938 |
|---|
Application
The anti-OAS1 Pab is used in Western blot to detect OAS1 in mouse liver lysate.
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with OAS1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
