WB
Western blot analysis of OAS1/3 using anti-OAS1/3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-OAS1/3 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
OAS1/3 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-OAS1/3 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
OAS1/3 was detected in a paraffin-embedded section of human liver tissue. The tissue section was incubated with rabbit anti-OAS1/3 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Product Name | Rabbit polyclonal antibody to OAS1/3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human OAS1 recombinant protein (Position: M1-L345) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | OAS1 |
|---|---|
| Gene Synonyms | OIAS IFI-4 OIASI IMD100 E18/E16 |
| Gene Full Name | 2'-5'-oligoadenylate synthetase 1 |
| Gene Summary | This interferon-induced gene encodes a protein that synthesizes 2',5'-oligoadenylates (2-5As). This protein plays a key role in innate cellular antiviral response, and has been implicated in other cellular processes like cell growth and apoptosis. Alternative splicing results in multiple transcript variants with different enzymatic activities. Polymorphisms in this gene have been associated with susceptibility to viral infection, including SARS-CoV-2, and diabetes mellitus, type 1. This gene is located in a cluster of related genes on chromosome 12. [provided by RefSeq, May 2022] |
| Alternative Names | (2 5)oligo(A) synthase 1 2 5 oligoadenylate synthase 1 2 5A synthase 1 E18/E16 IFI 4 OAS1 OAS1/3 OIAS OIASI p46/p42 OAS |
| Molecular Weight(MW) | 46 kDa(Observed MW 105 kDa) |
| Cellular Localization | Cytoplasm,Endoplasmic reticulum,Microsome,Mitochondrion,Nucleus,Secreted. |
WB
Western blot analysis of OAS1/3 using anti-OAS1/3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-OAS1/3 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
OAS1/3 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-OAS1/3 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
OAS1/3 was detected in a paraffin-embedded section of human liver tissue. The tissue section was incubated with rabbit anti-OAS1/3 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
