Application
A. Western blot analysis on (A) HeLa and (B) 293 cell lysate using anti-p16-INK4a RabMAb (Cat. #AJ1565a).Application
B. Immunofluorescent staining of HeLa cells using anti-p16/INK4a RabMAb (Cat. #AJ1565a).| Product Name | p16/INK4a Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDKN2A, CDKN2, MTS1, Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3, Multiple tumor suppressor 1 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP435Y |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CDKN2 MTS1 |
|---|---|
| Alternative Names | CDKN2A CDKN2 MTS1 Cyclin-dependent kinase inhibitor 2A isoforms 1/2/3 Multiple tumor suppressor 1 |
| Molecular Weight(MW) | 16533 Da |
| Function | Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein |
| Tissue Specificity | Widely expressed but not detected in brain or skeletal muscle. Isoform 3 is pancreas-specific |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 1029 |
|---|
Application
A. Western blot analysis on (A) HeLa and (B) 293 cell lysate using anti-p16-INK4a RabMAb (Cat. #AJ1565a).
Application
B. Immunofluorescent staining of HeLa cells using anti-p16/INK4a RabMAb (Cat. #AJ1565a).| Application Notes | WB:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
