Application
p16INK4a Antibody (C-term E119) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the p16INK4a antibody detected the p16INK4a protein (arrow).Application
p16INK4a Antibody (C-term E119) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of p16INK4a Antibody (C-term E119) for immunohistochemistry. Clinical relevance has not been evaluated.Application
p16INK4a Antibody (C-term E119) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of p16INK4a Antibody (C-term E119)(Cat#AP11690b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).| Product Name | p16INK4a Antibody (C-term E119) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDKN2A; CDKN2; MTS1; Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3; Cyclin-dependent kinase 4 inhibitor A; Multiple tumor suppressor 1; p16-INK4a |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CDKN2 MTS1 |
|---|---|
| Alternative Names | CDKN2A CDKN2 MTS1 Cyclin-dependent kinase inhibitor 2A isoforms 1/2/3 Cyclin-dependent kinase 4 inhibitor A Multiple tumor suppressor 1 p16-INK4a |
| Molecular Weight(MW) | 16533 Da |
| Function | Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein |
| Tissue Specificity | Widely expressed but not detected in brain or skeletal muscle. Isoform 3 is pancreas-specific |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 1029 |
|---|---|
| Nucleotide Accession | NP_478104.2 NP_000068.1 |
Application
p16INK4a Antibody (C-term E119) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the p16INK4a antibody detected the p16INK4a protein (arrow).
Application
p16INK4a Antibody (C-term E119) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of p16INK4a Antibody (C-term E119) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
p16INK4a Antibody (C-term E119) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of p16INK4a Antibody (C-term E119)(Cat#AP11690b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
