Application
PAOX Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PAOX antibody detected the PAOX protein (arrow).Application
PAOX Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the PAOX antibody detected the PAOX protein (arrow).Application
PAOX antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PAOX antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | PAOX Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PAOX; PAO; Peroxisomal N(1)-acetyl-spermine/spermidine oxidase; Polyamine oxidase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PAO |
|---|---|
| Alternative Names | PAOX PAO Peroxisomal N(1)-acetyl-spermine/spermidine oxidase Polyamine oxidase |
| Molecular Weight(MW) | 70290 Da |
| Function | Flavoenzyme which catalyzes the oxidation of N(1)- acetylspermine to spermidine and is thus involved in the polyamine back-conversion. Can also oxidize N(1)-acetylspermidine to putrescine. Substrate specificity: N(1)-acetylspermine = N(1)- acetylspermidine > N(1),N(12)-diacylspermine >> spermine. Does not oxidize spermidine. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs |
| Tissue Specificity | Widely expressed. Not detected in spleen. Expressed at lower level in neoplastic tissues |
| Cellular Localization | Peroxisome (By similarity). Cytoplasm (By similarity) |
| Entrez Gene | 196743 |
|---|---|
| Nucleotide Accession | NP_997010.1 NP_997011.1 NP_690875.1 |
Application
PAOX Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PAOX antibody detected the PAOX protein (arrow).
Application
PAOX Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the PAOX antibody detected the PAOX protein (arrow).
Application
PAOX antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PAOX antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
