Application
Figure 1: Western blot analysis using anti-PAR4 monoclonal antibody against full-length Trx-Par4 recombinant protein(1) and Hela cell lysate(2).Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human brain(left) and breast carcinoma(right), showing cytoplasmic and membrane localization using anti-PAR4 antibody with DAB staining.| Product Name | PAR4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PAWR |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3G9H7,4H12E9 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PAR4 |
|---|---|
| Alternative Names | PAWR |
| Molecular Weight(MW) | 36568 Da |
| Function | Pro-apoptopic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down- regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1 |
| Tissue Specificity | Widely expressed. Expression is elevated in various neurodegenerative diseases such as amyotrophic lateral sclerosis, Alzheimer, Parkinson and Huntington diseases and stroke. Down-regulated in several cancers |
| Cellular Localization | Cytoplasm. Nucleus. Note=Mainly cytoplasmic in absence of apoptosis signal and in normal cells. Nuclear in most cancer cell lines. Nuclear entry seems to be essential but not sufficient for apoptosis (By similarity). Nuclear localization includes nucleoplasm and PML nuclear bodies |
| Entrez Gene | 5074 |
|---|---|
| Nucleotide Accession | NP_002574.2 |
Application
Figure 1: Western blot analysis using anti-PAR4 monoclonal antibody against full-length Trx-Par4 recombinant protein(1) and Hela cell lysate(2).
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human brain(left) and breast carcinoma(right), showing cytoplasmic and membrane localization using anti-PAR4 antibody with DAB staining.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of PAR4(aa1-330) expressed in E. Coli. |
