Application
Figure 1: Western blot analysis using PARP mouse mAb against Jurkat (1), K562 (2), Hela (3), Raji (4),THP-1(5) and SW620(6) cell lysate.Application
Figure 2: Flow cytometric analysis of Jurkat cells using anti-PARP mAb (green) and negative control (purple).| Product Name | PARP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PARP; PPOL; ADPRT; ADPRT1; PARP-1; pADPRT-1; PARP1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 7A10 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500 - 2000. FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ADPRT PPOL |
|---|---|
| Alternative Names | PARP PPOL ADPRT ADPRT1 PARP-1 pADPRT-1 PARP1 |
| Molecular Weight(MW) | 113084 Da |
| Function | Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP- ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production |
| Tissue Specificity | Synthetic peptide of human PARP, conjugated to KLH. |
| Cellular Localization | Nucleus. Nucleus, nucleolus. |
| Entrez Gene | 142 |
|---|---|
| Nucleotide Accession | NP_001609.2 |
Application
Figure 1: Western blot analysis using PARP mouse mAb against Jurkat (1), K562 (2), Hela (3), Raji (4),THP-1(5) and SW620(6) cell lysate.
Application
Figure 2: Flow cytometric analysis of Jurkat cells using anti-PARP mAb (green) and negative control (purple).| Application Notes | WB:1:500 - 2000. FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Synthetic peptide of human PARP, conjugated to KLH. |
