Application
Figure 1: Western blot analysis using anti-PBEF1 monoclonal antibody against truncated PBEF1-His recombinant protein(1) and full-length GFP-PBEF1(aa1-491) transfected COS7 cell lysate(2).| Product Name | PBEF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | VF; PBEF; PBEF1; VISFATIN; NAMPT |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1D3A12 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500 - 2000. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PBEF PBEF1 |
|---|---|
| Alternative Names | VF PBEF PBEF1 VISFATIN NAMPT |
| Molecular Weight(MW) | 55521 Da |
| Function | Catalyzes the condensation of nicotinamide with 5- phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway (By similarity) |
| Tissue Specificity | Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues |
| Cellular Localization | Cytoplasm (By similarity). |
| Entrez Gene | 10135 |
|---|---|
| Nucleotide Accession | NP_005737.1 |
Application
Figure 1: Western blot analysis using anti-PBEF1 monoclonal antibody against truncated PBEF1-His recombinant protein(1) and full-length GFP-PBEF1(aa1-491) transfected COS7 cell lysate(2).| Application Notes | WB:1:500 - 2000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of PBEF1(aa338-479) expressed in E. Coli. |
