Application
The anti-PDGFA (N-term) is used in Western blot to detect PDGFA in HL60 cell lysate.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PDGFA antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | PDGFA Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PDGFA; PDGF1; Platelet-derived growth factor subunit A; PDGF-1; Platelet-derived growth factor A chain; Platelet-derived growth factor alpha polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PDGF1 |
|---|---|
| Alternative Names | PDGFA PDGF1 Platelet-derived growth factor subunit A PDGF-1 Platelet-derived growth factor A chain Platelet-derived growth factor alpha polypeptide |
| Molecular Weight(MW) | 24043 Da |
| Function | Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB (By similarity) |
| Tissue Specificity | This PDGFA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from amino acids 60-90 of human PDGFA. |
| Cellular Localization | Secreted. Note=Released by platelets upon wounding |
Application
The anti-PDGFA (N-term) is used in Western blot to detect PDGFA in HL60 cell lysate.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PDGFA antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
