Application
Western blot analysis of PDK3 Antibody in MCF-7 cell line lysates (35ug/lane). PDK3 (arrow) was detected using the purified Pab.Application
PDK3 Antibody FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PDK3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PDK3; PDHK3; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3, mitochondrial; Pyruvate dehydrogenase kinase isoform 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PDHK3 |
|---|---|
| Alternative Names | PDK3 PDHK3 [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3 mitochondrial Pyruvate dehydrogenase kinase isoform 3 |
| Molecular Weight(MW) | 46939 Da |
| Function | Inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. Can also phosphorylate PDHA2. Decreases glucose utilization and increases fat metabolism in response to prolonged fasting, and as adaptation to a high-fat diet. Plays a role in glucose homeostasis and in maintaining normal blood glucose levels in function of nutrient levels and under starvation. Plays a role in the generation of reactive oxygen species |
| Tissue Specificity | Found almost exclusively in heart and skeletal muscle |
| Cellular Localization | Mitochondrion matrix. |
| Entrez Gene | 5165 |
|---|
Application
Western blot analysis of PDK3 Antibody in MCF-7 cell line lysates (35ug/lane). PDK3 (arrow) was detected using the purified Pab.
Application
PDK3 Antibody FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
