Application
Western blot analysis of PDK3 (arrow) using rabbit polyclonal PDK3 Antibody (N-term) (Cat# AP7040a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDK3 gene (Lane 2) (Origene Technologies).Application
PDK3 Antibody (M1) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the PDK3 antibody detected the PDK3 protein (arrow).| Product Name | PDK3 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PDK3; PDHK3; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3, mitochondrial; Pyruvate dehydrogenase kinase isoform 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PDHK3 |
|---|---|
| Alternative Names | PDK3 PDHK3 [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3 mitochondrial Pyruvate dehydrogenase kinase isoform 3 |
| Molecular Weight(MW) | 46939 Da |
| Function | Inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. Can also phosphorylate PDHA2. Decreases glucose utilization and increases fat metabolism in response to prolonged fasting, and as adaptation to a high-fat diet. Plays a role in glucose homeostasis and in maintaining normal blood glucose levels in function of nutrient levels and under starvation. Plays a role in the generation of reactive oxygen species |
| Tissue Specificity | Found almost exclusively in heart and skeletal muscle |
| Cellular Localization | Mitochondrion matrix. |
| Entrez Gene | 5165 |
|---|
Application
Western blot analysis of PDK3 (arrow) using rabbit polyclonal PDK3 Antibody (N-term) (Cat# AP7040a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDK3 gene (Lane 2) (Origene Technologies).
Application
PDK3 Antibody (M1) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the PDK3 antibody detected the PDK3 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
