Dot blot analysis of anti-Phospho-APG8b(MAP1LC3B)-T29 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
MAP1LC3B MAP1ALC3 Microtubule-associated proteins 1A/1B light chain 3B Autophagy-related protein LC3 B Autophagy-related ubiquitin-like modifier LC3 B MAP1 light chain 3-like protein 2 MAP1A/MAP1B light chain 3 B Microtubule-associated protein 1 lig
Molecular Weight(MW)
14688 Da
Function
Involved in formation of autophagosomal vacuoles (autophagosomes)
Tissue Specificity
Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver
Cellular Localization
Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes
Dot blot analysis of anti-Phospho-APG8b(MAP1LC3B)-T29 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
Application Notes
DB:1:100~500
Additional Information
Form
Liquid
Storage Instructions
For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.