Application
APG8b(MAP1LC3B) Antibody (T29) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the APG8b(MAP1LC3B) antibody detected the APG8b(MAP1LC3B) protein (arrow).Application
APG8b(MAP1LC3B) Antibody (T29) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the APG8b(MAP1LC3B) antibody detected the APG8b(MAP1LC3B) protein (arrow).Application
APG8b(MAP1LC3B) Antibody (N-term T29) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of APG8b(MAP1LC3B) Antibody (N-term T29) for immunohistochemistry. Clinical relevance has not been evaluated.Application
APG8b(MAP1LC3B) Antibody (N-term T29) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | APG8b(MAP1LC3B) Antibody (N-term T29) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAP1LC3B; MAP1ALC3; Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; Microtubule-associated protein 1 lig |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MAP1ALC3 |
|---|---|
| Alternative Names | MAP1LC3B MAP1ALC3 Microtubule-associated proteins 1A/1B light chain 3B Autophagy-related protein LC3 B Autophagy-related ubiquitin-like modifier LC3 B MAP1 light chain 3-like protein 2 MAP1A/MAP1B light chain 3 B Microtubule-associated protein 1 lig |
| Molecular Weight(MW) | 14688 Da |
| Function | Involved in formation of autophagosomal vacuoles (autophagosomes) |
| Tissue Specificity | Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver |
| Cellular Localization | Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes |
| Entrez Gene | 81631 |
|---|---|
| Nucleotide Accession | NP_073729.1 |
Application
APG8b(MAP1LC3B) Antibody (T29) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the APG8b(MAP1LC3B) antibody detected the APG8b(MAP1LC3B) protein (arrow).
Application
APG8b(MAP1LC3B) Antibody (T29) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the APG8b(MAP1LC3B) antibody detected the APG8b(MAP1LC3B) protein (arrow).
Application
APG8b(MAP1LC3B) Antibody (N-term T29) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of APG8b(MAP1LC3B) Antibody (N-term T29) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
APG8b(MAP1LC3B) Antibody (N-term T29) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
