Application
Western blot analysis of APG8b (MAP1LC3B) Antibody (T93/Y99) in MCF-7 cell line lysates (35ug/lane). MAP1LC3B (arrow) was detected using the purified Pab.Application
APG8b (MAP1LC3B)-T93/Y99 western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the MAP1LC3B antibody detected MAP1LC3B protein (arrow).Application
APG8b (MAP1LC3B)-T93/Y99 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the APG8b (MAP1LC3B)-T93/Y99 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
APG8b (MAP1LC3B)-T93/Y99 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | APG8b (MAP1LC3B)-T93/Y99 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAP1LC3B; MAP1ALC3; Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; Microtubule-associated protein 1 lig |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MAP1ALC3 |
|---|---|
| Alternative Names | MAP1LC3B MAP1ALC3 Microtubule-associated proteins 1A/1B light chain 3B Autophagy-related protein LC3 B Autophagy-related ubiquitin-like modifier LC3 B MAP1 light chain 3-like protein 2 MAP1A/MAP1B light chain 3 B Microtubule-associated protein 1 lig |
| Molecular Weight(MW) | 14688 Da |
| Function | Involved in formation of autophagosomal vacuoles (autophagosomes) |
| Tissue Specificity | Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver |
| Cellular Localization | Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes |
| Entrez Gene | 81631 |
|---|---|
| Nucleotide Accession | NP_073729.1 |
Application
Western blot analysis of APG8b (MAP1LC3B) Antibody (T93/Y99) in MCF-7 cell line lysates (35ug/lane). MAP1LC3B (arrow) was detected using the purified Pab.
Application
APG8b (MAP1LC3B)-T93/Y99 western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the MAP1LC3B antibody detected MAP1LC3B protein (arrow).
Application
APG8b (MAP1LC3B)-T93/Y99 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the APG8b (MAP1LC3B)-T93/Y99 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
APG8b (MAP1LC3B)-T93/Y99 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
