Application
Dot blot analysis of Phospho-ATF2-T51 polyclonal antibody (Cat# AP3334a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.| Product Name | Phospho-ATF2-T51 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-T51 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CREB2, CREBP1 |
|---|---|
| Alternative Names | ATF2 CREB2 CREBP1 Cyclic AMP-dependent transcription factor ATF-2 Activating transcription factor 2 Cyclic AMP-responsive element-binding protein 2 HB16 cAMP response element-binding protein CRE-BP1 |
| Molecular Weight(MW) | 54537 Da |
| Function | Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'- GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex |
| Tissue Specificity | Abundant expression seen in the brain. |
| Cellular Localization | Nucleus. |
Application
Dot blot analysis of Phospho-ATF2-T51 polyclonal antibody (Cat# AP3334a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.| Application Notes | DB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
