Application
Dot blot analysis of anti-Phospho-ATXN1-pT236 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.Application
Confocal immunofluorescent analysis of Phospho-ATXN1-pT236 Antibody(Cat#AP3568a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).| Product Name | Phospho-ATXN1-pT236 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-T236 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DBIF |
| DB:1:500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ATX1, SCA1 |
|---|---|
| Alternative Names | ATXN1 ATX1 SCA1 Ataxin-1 Spinocerebellar ataxia type 1 protein |
| Molecular Weight(MW) | 86923 Da |
| Function | Chromatin-binding factor that repress Notch signaling in the absence of Notch intracellular domain by acting as a CBF1 corepressor. Binds to the HEY promoter and might assist, along with NCOR2, RBPJ-mediated repression. Binds RNA in vitro. May be involved in RNA metabolism. The expansion of the polyglutamine tract may alter this function |
| Tissue Specificity | Widely expressed throughout the body. |
| Cellular Localization | Cytoplasm (By similarity). Nucleus. Note=Colocalizes with USP7 in the nucleus |
Application
Dot blot analysis of anti-Phospho-ATXN1-pT236 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Application
Confocal immunofluorescent analysis of Phospho-ATXN1-pT236 Antibody(Cat#AP3568a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).| Application Notes | DB:1:500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. |
