Application
Dot blot analysis of anti-BRAF Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Product Name | Phospho-BRAF-pT400 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-T400 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BRAF1, RAFB1 |
|---|---|
| Alternative Names | BRAF BRAF1 RAFB1 Serine/threonine-protein kinase B-raf Proto-oncogene B-Raf p94 v-Raf murine sarcoma viral oncogene homolog B1 |
| Molecular Weight(MW) | 84437 Da |
| Function | Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron |
| Tissue Specificity | Brain and testis. |
| Cellular Localization | Nucleus (By similarity). Cytoplasm. Cell membrane (By similarity). Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes (By similarity) |
| Entrez Gene | 673 |
|---|
Application
Dot blot analysis of anti-BRAF Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Application Notes | DB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. |
