Application
A. Western blot analysis on PC-12 cell lysates using anti-Phospho-B-Raf (p401) RabMAb (Cat. #AJ1106b), 1:5000 dilution. Cells were either (A) untreated (B) treated with TPA.| Product Name | B-Raf Antibody Phospho (pT401) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-T401 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR2208Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000~5000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BRAF1, RAFB1 |
|---|---|
| Alternative Names | BRAF BRAF1 RAFB1 B-Raf proto-oncogene serine/threonine-protein kinase p94 v-Raf murine sarcoma viral oncogene homolog B1 |
| Molecular Weight(MW) | 84437 Da |
| Function | Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron |
| Tissue Specificity | Brain and testis. |
| Cellular Localization | Nucleus (By similarity). Cytoplasm. Cell membrane (By similarity). Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes (By similarity) |
| Entrez Gene | 673 |
|---|
Application
A. Western blot analysis on PC-12 cell lysates using anti-Phospho-B-Raf (p401) RabMAb (Cat. #AJ1106b), 1:5000 dilution. Cells were either (A) untreated (B) treated with TPA.| Application Notes | WB:1:1000~5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
