Application
Dot blot analysis of phospho-BRAF-T439 polyclonal antibody(Cat# AP3303a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.| Product Name | Phospho-BRAF-T439 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-T439 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:0.388888888889 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BRAF1, RAFB1 |
|---|---|
| Alternative Names | BRAF BRAF1 RAFB1 Serine/threonine-protein kinase B-raf Proto-oncogene B-Raf p94 v-Raf murine sarcoma viral oncogene homolog B1 |
| Molecular Weight(MW) | 84437 Da |
| Function | Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron |
| Tissue Specificity | Brain and testis. |
| Cellular Localization | Nucleus (By similarity). Cytoplasm. Cell membrane (By similarity). Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes (By similarity) |
| Entrez Gene | 673 |
|---|
Application
Dot blot analysis of phospho-BRAF-T439 polyclonal antibody(Cat# AP3303a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.| Application Notes | DB:0.388888888889 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
