Application
Western blot analysis of anti-Phospho-Caspase-pS257 in liver cell line lysates (35ug/lane). Casp6-pS257(arrow) was detected using the purified Pab (1:60 dilution).| Product Name | Phospho-Caspase 6-S257 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S257 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MCH2 |
|---|---|
| Alternative Names | CASP6 MCH2 Caspase-6 Apoptotic protease Mch-2 Caspase-6 subunit p18 Caspase-6 subunit p11 |
| Molecular Weight(MW) | 33310 Da |
| Function | Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death |
| Tissue Specificity | This Phospho-Caspase 6-S257 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S257 of human caspase 6. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 839 |
|---|
Application
Western blot analysis of anti-Phospho-Caspase-pS257 in liver cell line lysates (35ug/lane). Casp6-pS257(arrow) was detected using the purified Pab (1:60 dilution).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
