Application
The anti-Phospho-CDC6-S54 Pab is used in Western blot to detect Phospho-CDC6-S54 in Ramos tissue lysateApplication
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | Phospho-CDC6-S54 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S54 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CDC18L |
|---|---|
| Alternative Names | CDC6 CDC18L Cell division control protein 6 homolog CDC6-related protein Cdc18-related protein p62(cdc6) |
| Molecular Weight(MW) | 62720 Da |
| Function | Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated |
| Tissue Specificity | This Phospho-CDC6-S54 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S54 of human Cdc6. |
| Cellular Localization | Nucleus. Cytoplasm. Note=The protein is nuclear in G1 and cytoplasmic in S-phase cells |
| Entrez Gene | 990 |
|---|
Application
The anti-Phospho-CDC6-S54 Pab is used in Western blot to detect Phospho-CDC6-S54 in Ramos tissue lysate
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
