Application
A.Western blot analysis on (A) Raji and (B) Raji + FBS cell lysates using anti-Phospho-Cdc6 (pS54) RabMAb (Cat. #AJ1176b), 1:2000 dilution. Cells were either (A) untreated (B) treated with FBSApplication
B. Immunofluorescent staining of HeLa cells using Phospho-specific anti-CDC6 RabMAb (Cat. #AJ1176b).| Product Name | Cdc6 Antibody Phospho (pS54) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDC6, CDC18L, Cell division control protein 6 homolog, CDC6-related protein;p62(cdc6) |
| Modification | p-S54 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR759Y |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:1000~2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CDC18L |
|---|---|
| Alternative Names | CDC6 CDC18L Cell division control protein 6 homolog CDC6-related protein p62(cdc6) |
| Molecular Weight(MW) | 62720 Da |
| Function | Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated |
| Tissue Specificity | A phospho-specific peptide corresponding to residues surrounding serine 54 of human Cdc6 was used as an immunogen. The antibody only detects Cdc6 phosphorylated on Serine 54. |
| Cellular Localization | Nucleus. Cytoplasm. Note=The protein is nuclear in G1 and cytoplasmic in S-phase cells |
| Entrez Gene | 990 |
|---|
Application
A.Western blot analysis on (A) Raji and (B) Raji + FBS cell lysates using anti-Phospho-Cdc6 (pS54) RabMAb (Cat. #AJ1176b), 1:2000 dilution. Cells were either (A) untreated (B) treated with FBS
Application
B. Immunofluorescent staining of HeLa cells using Phospho-specific anti-CDC6 RabMAb (Cat. #AJ1176b).| Application Notes | WB:1:1000~2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
