Application
Western blot analysis of extracts from COLO205 cells using Cofilin Phospho-Cofilin-S3 Antibody (Cat#AE1009b)(Lane 2)and the same antibody preincubated with blocking peptide(Lane 1).Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-Cofilin-S3 Antibody (Cat#AE1009b).Application
Immunofluorescence staining of methanol-fixed HeLa cells using Phospho-Cofilin-S3 Antibody| Product Name | Phospho-Cofilin-S3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:500~1:1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CFL |
|---|---|
| Alternative Names | 18 kDa phosphoprotein p18 Cofilin non-muscle isoform Cofilin-1 |
| Molecular Weight(MW) | 18502 Da |
| Function | Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization |
| Tissue Specificity | Widely distributed in various tissues. |
| Cellular Localization | Nucleus matrix. Cytoplasm, cytoskeleton. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium membrane; Peripheral membrane protein; Cytoplasmic side. Note=Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide |
| Entrez Gene | 1072 |
|---|
Application
Western blot analysis of extracts from COLO205 cells using Cofilin Phospho-Cofilin-S3 Antibody (Cat#AE1009b)(Lane 2)and the same antibody preincubated with blocking peptide(Lane 1).
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-Cofilin-S3 Antibody (Cat#AE1009b).
Application
Immunofluorescence staining of methanol-fixed HeLa cells using Phospho-Cofilin-S3 Antibody| Application Notes | WB:1:500~1:1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | affinity Purified IgG, in PBS, 0.02% sodium azide and 50% glycerol. |
