Application
Western blot analysis of Cofilin (arrow) using rabbit polyclonal Phospho-Cofilin (Ser3) antibody.crl . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Cofilin gene (Lane 2) .Application
Cofilin antibody (Ser3) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Cofilin antibody (Ser3) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Cofilin (Ser3) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CFL1; CFL; Cofilin-1; 18 kDa phosphoprotein; Cofilin, non-muscle isoform |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CFL |
|---|---|
| Alternative Names | CFL1 CFL Cofilin-1 18 kDa phosphoprotein Cofilin non-muscle isoform |
| Molecular Weight(MW) | 18502 Da |
| Function | Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization |
| Tissue Specificity | Widely distributed in various tissues. |
| Cellular Localization | Nucleus matrix. Cytoplasm, cytoskeleton. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium membrane; Peripheral membrane protein; Cytoplasmic side. Note=Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide |
| Entrez Gene | 1072 |
|---|---|
| Nucleotide Accession | NP_005498.1 |
Application
Western blot analysis of Cofilin (arrow) using rabbit polyclonal Phospho-Cofilin (Ser3) antibody.crl . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Cofilin gene (Lane 2) .
Application
Cofilin antibody (Ser3) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Cofilin antibody (Ser3) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
